TY - JOUR AU - Boiko, Sergiy AU - Netsvetov, Maksym AU - Radchenko, Vladimir PY - 2023/05/04 Y2 - 2024/03/28 TI - Cellulose biosaccharification by Irpex lacteus wood decay fungus JF - Maderas-Cienc Tecnol JA - Maderas-Cienc Tecnol VL - 25 IS - SE - Article DO - 10.4067/s0718-221x2023000100435 UR - https://revistas.ubiobio.cl/index.php/MCT/article/view/5912 SP - 1-12 AB - <p>Enzymatic hydrolysis is an environmentally friendly technology to produce sugars from pretreated biomass. Here, we show that the new Il-11 <em>Irpex lacteus</em> strain can synthesize cellulases in a high quantity. The peptone and filter paper contained in the medium significantly enhanced activity of endo-1,4-<em>β</em>-D-glucanases (app. 50 IU/mL) and total cellulases (app. 9 IU/mL), whereas the medium with peptone and sodium carboxymethyl cellulose stimulated activity of exo-1,4-<em>β</em>-D-glucanases (33 IU/mL). The expression of cellulases reached its maximum within 96–144 hours, and the optimum pH is 3,7. Thermal treatment at 30 °C for 60 minutes activated endo-1,4-<em>β</em>-D-glucanases and total cellulases, while exo-1,4-<em>β</em>-D-glucanases activity was enhanced following 40 °C treatment. In total, the cellulases complex (300 IU/g) saccharified untreated cellulose by 38 % in 48 hours. Concentrate with filter paper activity 100 IU/g is the more balanced enzyme-substrate ratio (2 %), which allows prolonging the saccharification process that will have a positive effect on the cost of the final product.</p> ER -